-2011 EN/Wada & Kobayashi Lab

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

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Original Article


Kobayashi, K., Ohnishi, A., Sasaki, D., Fujii, S., Iwase, A., Sugimoto, K., Masuda, T. and Wada, H. (2017) Shoot removal induces chloroplast development in roots via cytokinin signaling. Plant Physiol. 173: 2340-2355.

Petereit, J., Katayama, K., Lorenz, C., Ewert, L., Schertl, P., Kitsche, A., Wada, H., Frentzen, M., Braun, H.-P. and Eubel, H. (2017) Cardiolipin supports respiratory enzymes in plants in different ways. Front. Plant Sci. 8: 72.

Fujisawa, T., Narikawa, R., Maeda, S., Watanabe, S., Kanesaki, Y., Kobayashi, K., Nomata, J., Hanaoka, M., Watanabe, M., Ehira, S., Suzuki, E., Awai, K. and Nakamura, Y. (2017) CyanoBase: a large-scale update on its 20th anniversary. Nucleic Acids Res. 45: D551-D554.


Lin, Y.-C., Kobayashi, K., Hung, C.-H., Wada, H. and Nakamura, Y. (2016) Arabidopsis PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE1 (PGPP1) involved in phosphatidylglycerol biosynthesis and photosynthetic function. Plant J. 88: 1022-1037.

Endo, K., Kobayashi, K. and Wada, H. (2016) Sulfoquinovosyldiacylglycerol has an essential role in Thermosynechococcus elongatus BP-1 under phosphate-deficient conditions. Plant Cell Physiol. 57: 2461-2471.

Espinas, N.A., Kobayashi, K., Sato, Y., Mochizuki, N., Takahashi, K., Tanaka, R. and Masuda, T. (2016) Allocation of heme is differentially regulated by ferrochelatase isoforms in Arabidopsis cells. Front. Plant Sci. 7: 1326.

Kobayashi, K., Endo, K. and Wada, H. (2016) Multiple impacts of loss of plastidic phosphatidylglycerol biosynthesis on photosynthesis during seedling growth of Arabidopsis. Front. Plant Sci. 7:336.

Koskinen, S., Hakkila, K., Gunnelius, L., Kurkela, J., Wada, H. and Tyystjärvi, T. (2016) In vivo recruitment analysis and a mutant strain without any group 2 σ factors reveal roles of different σ factors in cyanobacteria. Mol. Microbiol. 99:43-54.


Hung, C.-H., Kobayashi, K., Wada, H. and Nakamura, Y. (2015) Functional specificity of cardiolipin synthase revealed by the isolation of a cardiolipin synthase CrCLS1 in Chlamydomonas reinhardtii. Front. Microbiol. 6:1542.

Hung, C.-H., Endo, K., Kobayashi, K., Nakamura, Y. and Wada, H. (2015) Characterization of Chlamydomonas reinhardtii phosphatidylglycerophosphate synthase in Synechocystis sp. PCC 6803. Front. Microbiol. 6:842.

Endo, K., Mizusawa, N., Shen, J.-R., Yamada, M., Tomo, T., Komatsu, H., Kobayashi, M., Kobayashi, K. and Wada, H. (2015) Site-directed mutagenesis of amino acid residues of D1 protein interacting with phosphatidylglycerol affects the function of plastoquinone QB in photosystem II. Photosynth. Res., 126: 385-397.

Hung, C.-H., Kobayashi, K., Wada, H. and Nakamura, Y. (2015) Isolation and characterization of a phosphatidylglycerophosphate phosphatase1, PGPP1, in Chlamydomonas reinhardtii. Plant Physiol. Biochem. 92:56-61.

Okazaki, K., Miyagishima, S. and Wada, H. (2015) Phosphatidylinositol 4-phosphate negatively regulates chloroplast division. Plant Cell 27:663-674.

Kobayashi, K., Fujii, S., Sato, M., Toyooka, K. and Wada, H. (2015) Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis. Plant Cell Rep. 34:631-642


Fujii S, Kobayashi K, Nakamura Y, Wada H (2014) Inducible knockdown of MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE1 reveals roles of galactolipids in organelle differentiation in Arabidopsis cotyledons. Plant Physiol. 166: 1436-1449.

Kobayashi K, Masuda T, Tajima N, Wada H, Sato N (2014) Molecular phylogeny and intricate evolutionary history of the three isofunctional enzymes involved in the oxidation of protoporphyrinogen IX. Genome Biol. Evol. 6: 2141-2155.

Ujihara T, Nagano M, Wada H, Mitsuhashi S (2014) Identification of a novel type of polyunsaturated fatty acid synthase involved in the biosynthesis of arachidonic acid. FEBS Lett. 588: 4032-4036.

Hori K et al. (2014) Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation. Nat. Comun. 5: 3978.

Murakawa M, Shimojima M, Shimomura Y, Kobayashi K, Awai K, Ohta H (2014) Monogalactosyldiacylglycerol synthesis in the outer envelope membrane of chloroplasts is required for enhanced growth under sucrose supplementation. Front. Plant Sci. 5: 280.

Kobayashi K, Fujii S, Sasaki D, Baba S, Ohta H, Masuda T, Wada H (2014) Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis. Front. Plant Sci. 5: 272.

Gunnelius, L., Hakkila, K., Kurkela, J., Wada, H., Tyystjärvi, E. and Tyystjärvi, T. (2014) The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acid Res. 42: 4606-4614.

Yuzawa Y., Shimojima, M., Sato, R., Mizusawa, N., Yamamichi, K., Suzuki, M., Iwai, M., Hori, K., Wada, H., Masuda, S. and Ohta, H. (2014) Cyanobacterial monogalactosyldiacylglycerol-synthesis pathway is involved in normal unsaturation of galactolipids and low-temperature adaptation of Synechocystis sp. PCC 6803. Biochim. Biophys. Acta 1841:475-483.

Hakkila, K., Antal, T., Rehman, A. U., Kurkela, J., Wada, H., Vass, I., Tyystjärvi, E. and Tyystjärvi, T. (2014) Oxidative stress and photoinhibition can be separated in the cyanobacterium Synechocystis sp. PCC 6803. Biochim. Biophys. Acta 1837:217-225.

Tanoue R, Kobayashi M, Katayama K, Nagata N, Wada H (2014) Phosphatidylglycerol biosynthesis is required for the development of embryos and normal membrane structures of chloroplasts and mitochondria in Arabidopsis. FEBS Lett. 588: 1680-1685..


Kobayashi K, Sasaki D, Noguchi K, Fujinuma D, Komatsu H, Kobayashi M, Sato M, Toyooka K, Sugimoto K, Niyogi KK, Wada H, Masuda T (2013) Photosynthesis of root chloroplasts developed in Arabidopsis lines overexpressing GOLDEN2-LIKE transcription factors. Plant Cell Physiol. 54: 1365-77.

Kobayashi K, Narise T, Sonoike K, Hashimoto H, Sato N, Kondo M, Nishimura M, Sato M, Toyooka K, Sugimoto K, Wada H, Masuda T, Ohta H (2013) Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis. Plant J. 73: 250-261.

Sakata, S., Mizusawa, N., Kubota, H., Sakurai, I. and Wada H. (2013) Psb28 is involved in recovery of photosystem II at high temperature in Synechocystis sp. PCC 6803. Biochim. Biochys. Acta 1827: 50-59.


Chan, T., Shimizu, Y., Pospíšil, P., Nijo, N., Fujiwara, A., Taninaka, Y., Hori, H., Ishikawa, T., Nanba, D., Imai, A., Morita, N., Yoshioka-Nishimura, M., Izumi, Y., Yamamoto, Y., Kobayashi, H., Mizusawa, N., Wada, H. and Yamamoto, Y. (2012) Quality control of photosystem II. Lipid peroxidation causes subsidiary but substantial damage to photosystem II under illumination. PLoS ONE 7:e52100.

Kobayashi K, Obayashi T, Masuda T (2012) Role of the G-box element for regulation of chlorophyll biosynthesis in Arabidopsis root. Plant Signal Behav. 7: 922-926.

Espinas A.N, Kobayashi K, Takahashi S, Mochizuki N, Masuda T (2012) Evaluation of unbound free heme in plant cells by differential acetone extraction. Plant Cell Physiol. 53: 1344-1354.

Hedtke B, Alawady A, Albacete A, Kobayashi K, Melzer M, Roitsch T, Masuda T, Grimm B. (2012) Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis by impaired cytokinin metabolism. Plant Mol. Biol. 78: 77-93.

Kobayashi K, Baba S, Obayashi T, Sato M, Toyooka K, Keranen M, Aro E.M, Fukaki H, Ohta H, Sugimoto K, Masuda T. (2012) Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis. Plant Cell, 24: 1081-1095.

Narita, M., Mochizuki, A., Wada, H., Itsuno, Y. and Itsuno, S. (2012) The statistical study of peptide sequence for local secondary structure: Relative formation abilities (RFA) of local sequence for secondary structure units. Submitted.

Katayama, K and Wada, H. (2012) T-DNA insertion in the CLS gene for cardiolipin synthase affects development of Arabidopsis thaliana. Cytologia. 77: 123-129.


Watanabe, M., Kubota, H., Wada, H., Narikawa, R. and Ikeuchi, M. (2011) Novel supercomplex organization of Photosystem I in Anabaena and Cyanophora paradoxa. Plant Cell Physiol. 52:162-168.

Reviews, Proceedings, & Books

Kobayashi, K. and Masuda, T. (2016) Transcriptional regulation of tetrapyrrole biosynthesis in Arabidopsis thaliana. Front. Plant Sci. 7: 1811.

Kobayashi, K. (2017) Role of membrane glycerolipids in photosynthesis, thylakoid biogenesis and chloroplast development. J. Plant Res. 129: 565–580.

Kobayashi, K. and Wada, H. (2016) Roles of lipids in chloroplast biogenesis. In Lipids in plant and algae development. Edited by Nakamura, Y. and Li-Beisson, Y. pp. 103-125, Springer, Dordrecht.

Kobayashi, K., Endo, K. and Wada. H. (2016) Roles of lipids in photosynthesis. In Lipids in plant and algae development. Edited by Nakamura, Y. and Li-Beisson, Y. pp. 21-49, Springer, Dordrecht.

Okazaki, K., Miyagishima, S. and Wada, H. (2016) Preparation of chloroplast lipid membrane and lipid-protein interaction assay. Bioprotocol 6:e1720.

Okazaki, K., Miyagishima, S. and Wada, H. (2016) Measurement of PI4P levels in intact chloroplasts isolated from Arabidopsis thaliana. Bioprotocol 6:e1719.

Kobayashi, K. and Masuda, T. (2016) Regulation of chlorophyll metabolism in plants. In The 3rd Edition of the Handbook of Photosynthesis. Edited by Pessarakli, M. pp. 173-192, CRC Press. USA.

Listiawan, D.A., Tanoue, R., Kobayashi, K. and Masuda, T. (2015) Expression Analysis of Transcription Factors Involved in Chloroplast Differentiation. Procedia Chemistry 14, 146-151.

Mizusawa, N., Sakata, S., Sakurai, I., Kubota, H., Sato, N. and Wada, H. (2013) Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp. PCC 6803 under high-temperature stress. In Photosynthesis Research for Food, Fuel and Future. Edited by Kuang, T., Lu, C. and Zhang, L. pp. 620-624, Springer, Dordrecht.

Kobayashi, K. and Masuda, T. (2013) Spatial and temporal regulation of chloroplast development in Arabidopsis roots. In Photosynthesis Research for Food, Fuel and Future. Edited by Kuang, T., Lu, C. and Zhang, L. pp. 389-393, Springer, Dordrecht.

Mizusawa, N. and Wada, H. (2012) The role of lipids in photosystem II. Biochim. Biophys. Acta 1817:194-208.

Tanaka, R, Kobayashi, K, Masuda, T (2011) Tetrapyrrole metabolism in Arabidopsis thaliana. The Arabidopsis Book 9: e0145.

Kobayashi K, Masuda T (2011) Tetrapyrrole biosynthesis in plant systems. In K. Kadish et al., eds, Handbook of Porphyrin Science, World Scientific, 20: 143-198.